Director/Senior Principal Scientist, mRNA Therapeutics
Kernal Biologics, Inc., United States
Job Description
Kernal is creating mRNA drugs that instruct specific cells in the body on how to make their own medicine. Messenger RNA technology has proven extremely useful in rapidly developing vaccines against COVID. Kernal is developing the next generation of mRNA therapy, called mRNA 2.0. It solves a critical problem of cancer cell selectivity that affects mRNA and oncolytic virus-based immuno-oncology drugs. Located in Cambridge, MA, Kernal received three awards from Amgen and NASA. With roots at MIT, Harvard, and Big Pharma, Kernal’s interdisciplinary team of M.D.s and PhDs previously built a successful Biotech company and has deep expertise in mRNA space.
Kernal is seeking an individual with deep expertise in the mRNA therapeutics and synthetic biology field to lead mRNA therapeutics development efforts. This person will report to the Head of Platform and work closely with computational biology and mRNA delivery teams to execute on company goals. In addition, this person will oversee and, when necessary, help with routine in vitro,ex vivo, and in vivo work. The successful candidate must be able to work in a fast-paced and highly dynamic startup environment. The ideal candidate will be an outstanding leader and a great team player with excellent communication skills and a practical, inspiring management style.
Responsibilities:
- Independently design, execute and analyze preclinical studies
- Work closely with computational biology and LNP teams to deliver on technical milestones
- Lead the team and guide the company’s mRNA strategy
- Work with senior management to expand the company’s Ramp;D team
- Stay up-to-date on pertinent literature and prepare technical reports and white papers for internal use
- Assist in I.P. filings and manuscript preparations
- Manage CROs and collaborate with academic partners for external preclinical studies
- Present scientific findings internally and externally at critical scientific conferences and industry events
- Design and construct DNA Template for in vitro transcription
- Analyze mRNA product quality via Nanodrop, Bioanalyzer, HPLC, etc.
- Develop purification methods for in vitro transcribed mRNA
- Test IVT mRNAs in vivo and in vitro
- Optimize the mRNA production processes
- Automate RNA synthesis and purification processes
- Independently design and execute experiments to develop and optimize processes for mRNA synthesis
- Contribute creatively and strategically to assay design, development, and process optimization.
- Collaborate closely with other computational and wet-lab scientists.
- Maintain thorough electronic lab notebooks and documentation records
- Deliver reproducible and impactful results under ambitious timelines, present results in cross-functional meetings to a broad audience, both internally and externally.
- Proactively evaluate new technologies, be versed with current literature, and implement new features and functionality into the mRNA platform.
- Execute and troubleshoot standard protocols, develop and adopt new protocols into practice, and query literature to incorporate additional assays as needed
- Collect, archive, process, and organize biological samples
- Identify and lead external research relationships with academic and commercial partners.
Requirements
- Ph.D. or M.D., plus a minimum of 3 years (post-degree) industrial experience conducting cutting-edge research in Biological Engineering, Molecular Biology, Biochemistry, Microbiology, Chemistry, or related field, with specialization in mRNA therapeutics
- Deep knowledge of synthetic biology and mRNA therapeutics
- Expertise in RNA vector design, plasmid engineering, in vitro transcription, RNA purification, and transfection in mammalian systems
- Advanced knowledge and significant experience with primer design, DNA isolation, and DNA assembly, seamless cloning,
- Experience with diverse molecular biology and microbiology techniques, including DNA/RNA analysis, DNA, RNA, and protein purification, western blot, PCR, qRT-PCR, ELISA.
- Experience with animal studies, mammalian cell culture, and analysis of protein expression and gene reporter systems with various techniques as confocal microscopy, flow cytometry, cell luminescence, imagine systems, and quantitative data analysis
- Experience with automation
- Experience with nucleic acid purification and chemistry, enzyme kinetics, and analytical characterization of biomolecules
- Experience with analytical techniques HPLC, MALDI-TOF MS, LC-MS/MS, NMR a plus
- Experience with bioprocess engineering principles, process modeling, and Design of Experiment (DoE) statistical tools and GMP regulations
- Creative, innovative, proble